Human Finishing

The Human Genome Project (HGP), an international research effort to determine the complete genetic blueprint of a human being, was completed in April of 2003.

With funding from the Department of Energy, the Stanford Human Genome Center was responsible for finishing human chromosomes 5, 16 and 19, in total almost 11% of the human genome.

This project was completed in collaboration with the Joint Genome Institute (JGI) in Walnut Creek, CA. After the selection of large insert clones for sequencing, shotgun libraries were produced at the JGI and the clones drafted to an average depth of 10x. Shotgun data, subclone libraries and the large insert glycerol stock were then transferred to the SHGC, where the draft data was finished to meet the high quality standard adapted by the HGP. A minimal tiling set of finished large insert clones was then assembled producing the complete sequence of the 3 chromosomes.